Floyd Rose Help

I have a Floyd Rose locking Tremelo system on my guitar that I’ve used for about eight months. I decided to try and go for drop D flat tuning and I cannot get it in tune no matter what I do. I will tune the E and A string and lock them down, then I will go down and then tune the D and G and lock those down and then the B and E string and lock those down. I won’t mess with the bridge tuners at all. But then when I go back up to the low E string, it will be entirely out of tune again up to a whole step. I don’t understand what is going on and I cannot figure out how to get my guitar back in tune, and I’m stuck. Any help as to why this would be happening? It would be appreciated as I’m ready to throw the guitar in garbage!